Yoshimasa Makita1, Yasuhiro Imamura2, Kazuya Masuno3, Isao Tamura4, Shin-ichi Fujiwara1, Gotaro Shiota5, Akihiko Shiba6 and Pao-Li Wang7*

1Department of Chemistry, Osaka Dental University, Osaka, Japan
2Department of Pharmacology, Matsumoto Dental University, Nagano, Japan
3Department of Innovation in Dental Education, Osaka Dental University, Osaka, Japan
4Department of Oral Anatomy, Osaka Dental University, Osaka, Japan
5VMC Co., Ltd., Tokyo, Japan (currently Mediplas Pharmaceuticals)
6Department of Prosthodontics, Faculty of Dentistry, Showa University, Tokyo, Japan
7Department of Bacteriology, Osaka Dental University, Osaka, Japan

Abstract

Ozone is currently being considered as a possible oral antiseptic agent because it is strongly antimicrobial and does not induce microbial resistance. In the article, we examined the effects of ozone exposure on the production of collagen type-1 and inflammatory cytokines in primary human gingival fibroblasts (HGFs) in vitro using enzyme-linked immunosorbent assays. The addition of 0.5 ppm ozone significantly enhanced collagen type-1 production by HGFs within 24 h. Secretion of the pro-inflammatory cytokines interleukin-6 (IL-6) and IL-8 by HGFs treated with lipopolysaccharide decreased when ozone was present in the medium. Together, these results suggest that clinical use of ozone would facilitate the positive balance between HGF-mediated periodontal tissue maintenance

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