New ways to reduce facial age spots. Japanese Pharmaceutical R&D company Mediplus Pharma found the new ability of “High performance Glycerin” Effective in 92% of people after 8 weeks of continuous use.


Mediplus Pharma, Inc. (CEO: Kenji Ito, Head Office: Shibuya-ku, Tokyo,JAPAN) confirmed that cosmetics containing its patented ingredient, high performance ozonized glycerin, have the effect of reducing melanin (age spots) in the epidermis, and filed a new application patent which claimed melanin decomposition.

Before/ After (at 45 days)
  1. After 8 weeks of continuous use of a sample containing ozonized glycerin in clinical study, Reducing melanin in the epidermis in about 92% of users compared to before use* Melanin content decreased by an average of 20%*²
  2. By continuing to use cosmetics containing ozonized glycerin in clinical study, Confirmed the effect of reducing age spots (senile lentigos ) on the facial skin.
  3. Ozonized glycerin can directly decompose melanin*³ in vitro.

* As a result of applying a sample product containing ozonized glycerin to the half of the face of two pushes of the container in the morning and evening for 8 weeks, and measuring the density of melanin at the age spots using a Mexameter (melanin measuring instrument).
*² Rate of change after continuous use for 8 weeks based on melanin measurements in the inner part of the upper arm
*³The study was performed in vitro, not data on human skin. The effects on human skin are currently under investigation.

Mechanism of production of age spots(melanin)

The skin consists of the epidermis and the dermis, the epidermis consists of four layers: the stratum corneum, the granule layer, the spine layer, and the basal layer, in order from the surface. Melanin is produced by pigment cells (melanocytes) located in the basal layer between the epidermis and the dermis and is transported to nearby epidermal cells. Age spots are formed by the accumulation of melanin in the epidermis and are caused by prolonged exposure to UV light or by disturbances in the metabolic rate (turnover) of the skin.

Fig. 1. Mechanism of production of age spots (melanin)
  • (1) Decreased melanin surfaced in 92% of people*, melanin readings decreased by an average of 20%*²

Using cosmetic products containing ozonized glycerin, we verified the effect on surfaced age spots and conducted effects on melanin in human clinical trials.

【Test Method】

Using cosmetic products containing ozonized glycerin and glycerin without ozonized glycerin (hereinafter referred to as a control product), two pushes in the morning and evening are applied to the half face twice a day everyday. Four or eight weeks later, we photographed facial images with VISIA-Evolution and measured melanin content using a mexameter (melanin measuring instrument).
[Seven-O-One Research Co., Ltd. Survey conducted by 24 women aged 42-59 years old (12th April-7th June 2021)]

Comparison of decreased melanin levels at 8 weeks
changes in melanin levels over time
【Test Results】

It was confirmed that melanin levels decreased on the skin surface of about 92% of those who continued to use ozonized glycerin-containing samples for 8 weeks (Fig. 2). After 8 weeks of continuous use of samples containing ozonized glycerin, it was confirmed that the melanin value decreased by about 20% on average (Fig. 3).

  • (2) Effect of reducing age spots(senile lentigos) on the facial skin

Using a sample containing ozonized glycerin, we confirmed that it is also effective for age spots(senile stains) on the skin surface that have already appeared for a long time.

Verification of the effect on senile stains

Ozonized glycerin can be expected to work not only on the age spots reserves that will surface in the future, but also on “age spots” that have surfaced on the skin for a long time (Fig. 4).

  • (3) Melanin direct resolution*³ test of high performance ozonized glycerin

Continuous use of samples containing ozonized glycerin for 8 weeks has confirmed the effect of thinning age spots and blemishes in human clinical trials. Therefore, in order to elucidate the mechanism by which ozonized glycerin reduces age spots, we conducted tests to explore the possibility of “direct decomposition of melanin*³” of ozonized glycerin.

【Test Method】

A melanin solution was prepared and ozonized glycerin and five types of other skin blighting solvents were used to compare changes in melanin pigments in 1, 2, and 4 weeks.

Melanin direct dissolution test
melanin degradation activity by whitening ingredients
changes in melanin values of each ingredients
【Test Results】

Compared to five types (vitamin C, hydroquinone, kojic acid, niacinamide, tranexamic acid), which are mainly famous as blighting active ingredients, The ozonized glycerin has the effectiveness of Melanin direct dissolution *³” was confirmed (Figs. 5 and 6). It was confirmed that the concentration (depth) of melanin by 66% was reduced in 4 weeks (Fig. 7).

A hypothesis on the mechanism of lightening of age spot

Glycerin Double Actions
A. Promotes turnover and draws stagnant melanin to the skin surface
B. Directly breaks down melanin, the cause of age spot

the effects of ozonized glycerin as a lightening agent

The results suggest that ozonized glycerin formulations could lighten the age spots (melanin) to an advantage in 8 weeks, and that one of the mechanisms could be to directly decompose melanin already deposited in the facial skin(B).
(The results of the study “(A)Promoting turnover and pushing melanin up to surface” are foreseen in a separate release.)
“Ozonized glycerin”, which is extremely safe to the skin and has the possibility of direct decomposition of melanin, will become a next-generation skin care active ingredient that will attract more and more attention in the future.
Mediplus Pharma, Inc. continues to conduct research and development in order to contribute to new discoveries and the development of innovative products.