The Eff ect of Ozone Gel on Bone Matrix Production by Human Osteosarcoma Cell Line Saos-2

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Pao-Li Wang1)*, Yoichi Tachi2)*, Kazuya Masuno1), Nobutaka Okusa3) and Yasuhiro Imamura4)
1) Department of Innovation in Dental Education, Osaka Dental University, Osaka, Japan
2) Laboratory of Nutritional Physiology, Tokyo Kasei University, Tokyo, Japan
3) Department of Forensic Dentistry, Osaka Dental University, Osaka, Japan
4) Department of Dental Pharmacology, Matsumoto Dental University, Shiojiri, Japan
(Accepted for publication, April 20, 2018)

Abstract

Ozone is currently being considered as a potential oral antiseptic agent because it is highly antimicrobial and does not induce microbial resistance. In this study, we demonstrated that an optimal dosage of ozone gel enhanced the proliferation, type 1 collagen production, and alkaline phosphatase (ALP) secretion of Saos-2 cells in vitro. Proliferation of Saos-2 cells was assessed by MTT and DNA synthesis assays. Type 1 collagen production and ALP secretion were evaluated using enzymelinked immunosorbent assay (ELISA) and ALP assays. The cells were treated with/without 0.05, 0.5, 5 ppm ozone gel for 24 h. Ozone gel (0.5 ppm) signifi cantly induced the proliferation of Saos-2 cells. At this concentration, ozone gel enhanced type 1 collagen production and ALP secretion. The results indicated that ozone gel controls the cellular metabolism of osteoblasts, resulting in the secretion of early bone-related biomarkers.

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The Effect of Ozone on Collagen Type-1 and Inflammatory Cytokine Production in Human Gingival Fibroblasts

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Yoshimasa Makita1, Yasuhiro Imamura2, Kazuya Masuno3, Isao Tamura4, Shin-ichi Fujiwara1, Gotaro Shiota5, Akihiko Shiba6 and Pao-Li Wang7*

1Department of Chemistry, Osaka Dental University, Osaka, Japan
2Department of Pharmacology, Matsumoto Dental University, Nagano, Japan
3Department of Innovation in Dental Education, Osaka Dental University, Osaka, Japan
4Department of Oral Anatomy, Osaka Dental University, Osaka, Japan
5VMC Co., Ltd., Tokyo, Japan (currently Mediplas Pharmaceuticals)
6Department of Prosthodontics, Faculty of Dentistry, Showa University, Tokyo, Japan
7Department of Bacteriology, Osaka Dental University, Osaka, Japan

Abstract

Ozone is currently being considered as a possible oral antiseptic agent because it is strongly antimicrobial and does not induce microbial resistance. In the article, we examined the effects of ozone exposure on the production of collagen type-1 and inflammatory cytokines in primary human gingival fibroblasts (HGFs) in vitro using enzyme-linked immunosorbent assays. The addition of 0.5 ppm ozone significantly enhanced collagen type-1 production by HGFs within 24 h. Secretion of the pro-inflammatory cytokines interleukin-6 (IL-6) and IL-8 by HGFs treated with lipopolysaccharide decreased when ozone was present in the medium. Together, these results suggest that clinical use of ozone would facilitate the positive balance between HGF-mediated periodontal tissue maintenance

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Use of Ozone Gel in Various Fields Including Atopic Dermatitis and Its Future Application in Dentistry

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Background and Purpose:
In our country, it is estimated that approximately one in two people suffers from some form of allergy, with atopic dermatitis affecting about 10% of the population. Research into allergic diseases is gradually progressing, yet there are no complete preventive or curative treatments available. Current treatment primarily involves long-term symptomatic therapy through environmental management and pharmacotherapy with anti-inflammatory drugs. 1) Furthermore, it is also observed in the dentistry field with three-quarters of the population being said to be suffering from periodontal disease . The misuse of antibiotics, leading to multidrug-resistant bacteria, poses a significant social issue, and there are many cases where long-term antibiotic use is not feasible, especially in oral care. Both atopic dermatitis and periodontal disease are inflammatory diseases, and the long-term application of steroids as anti-inflammatory drugs has been problematic due to side effects like steroid-induced rosacea.

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