The Eff ect of Ozone Gel on Bone Matrix Production by Human Osteosarcoma Cell Line Saos-2
Pao-Li Wang1)*, Yoichi Tachi2)*, Kazuya Masuno1), Nobutaka Okusa3) and Yasuhiro Imamura4)
1) Department of Innovation in Dental Education, Osaka Dental University, Osaka, Japan
2) Laboratory of Nutritional Physiology, Tokyo Kasei University, Tokyo, Japan
3) Department of Forensic Dentistry, Osaka Dental University, Osaka, Japan
4) Department of Dental Pharmacology, Matsumoto Dental University, Shiojiri, Japan
(Accepted for publication, April 20, 2018)
Abstract
Ozone is currently being considered as a potential oral antiseptic agent because it is highly antimicrobial and does not induce microbial resistance. In this study, we demonstrated that an optimal dosage of ozone gel enhanced the proliferation, type 1 collagen production, and alkaline phosphatase (ALP) secretion of Saos-2 cells in vitro. Proliferation of Saos-2 cells was assessed by MTT and DNA synthesis assays. Type 1 collagen production and ALP secretion were evaluated using enzymelinked immunosorbent assay (ELISA) and ALP assays. The cells were treated with/without 0.05, 0.5, 5 ppm ozone gel for 24 h. Ozone gel (0.5 ppm) signifi cantly induced the proliferation of Saos-2 cells. At this concentration, ozone gel enhanced type 1 collagen production and ALP secretion. The results indicated that ozone gel controls the cellular metabolism of osteoblasts, resulting in the secretion of early bone-related biomarkers.
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The Effect of Ozone on Collagen Type-1 and Inflammatory Cytokine Production in Human Gingival Fibroblasts
Yoshimasa Makita1, Yasuhiro Imamura2, Kazuya Masuno3, Isao Tamura4, Shin-ichi Fujiwara1, Gotaro Shiota5, Akihiko Shiba6 and Pao-Li Wang7*
1Department of Chemistry, Osaka Dental University, Osaka, Japan
2Department of Pharmacology, Matsumoto Dental University, Nagano, Japan
3Department of Innovation in Dental Education, Osaka Dental University, Osaka, Japan
4Department of Oral Anatomy, Osaka Dental University, Osaka, Japan
5VMC Co., Ltd., Tokyo, Japan (currently Mediplas Pharmaceuticals)
6Department of Prosthodontics, Faculty of Dentistry, Showa University, Tokyo, Japan
7Department of Bacteriology, Osaka Dental University, Osaka, Japan
Abstract
Ozone is currently being considered as a possible oral antiseptic agent because it is strongly antimicrobial and does not induce microbial resistance. In the article, we examined the effects of ozone exposure on the production of collagen type-1 and inflammatory cytokines in primary human gingival fibroblasts (HGFs) in vitro using enzyme-linked immunosorbent assays. The addition of 0.5 ppm ozone significantly enhanced collagen type-1 production by HGFs within 24 h. Secretion of the pro-inflammatory cytokines interleukin-6 (IL-6) and IL-8 by HGFs treated with lipopolysaccharide decreased when ozone was present in the medium. Together, these results suggest that clinical use of ozone would facilitate the positive balance between HGF-mediated periodontal tissue maintenance
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Use of Ozone Gel in Various Fields Including Atopic Dermatitis and Its Future Application in Dentistry
Background and Purpose:
In our country, it is estimated that approximately one in two people suffers from some form of allergy, with atopic dermatitis affecting about 10% of the population. Research into allergic diseases is gradually progressing, yet there are no complete preventive or curative treatments available. Current treatment primarily involves long-term symptomatic therapy through environmental management and pharmacotherapy with anti-inflammatory drugs. 1) Furthermore, it is also observed in the dentistry field with three-quarters of the population being said to be suffering from periodontal disease . The misuse of antibiotics, leading to multidrug-resistant bacteria, poses a significant social issue, and there are many cases where long-term antibiotic use is not feasible, especially in oral care. Both atopic dermatitis and periodontal disease are inflammatory diseases, and the long-term application of steroids as anti-inflammatory drugs has been problematic due to side effects like steroid-induced rosacea.
The 36th Japanese Nursing Association Compendium: “Deodorization effects of ozone products against unpleasant cancer odor.”
Abstract:
The last stage of a human life is a crucial period for people and their family. Various approaches are being used to study the way people want to spend their last moments, including palliative care. In this study, we succeeded in drastically reducing the unpleasant cancer odor of a patient who had long been bothered by it by using ozone gel and ozone water. The treatment also cleansed the surface of the ulcer, suggesting that ozone gel might positively contribute to endstage medicine. This treatment suppressed the unpleasant odor and cleansed the patient’s body in her last stage, indicating that ozone gel might help people to spend their twilight years with dignity. With the hope of helping such patients, we intend to accelerate the development of medical products containing ozone gel and make them accessible as soon as possible. This case report summarizes an article presented at the 36th Japanese Nursing Association Compendium–General Nursing published by the Japanese Nursing Association on November 24, 2005.
The effect of ozone water on extensive burns
1. Yagi Hospital, Department of Surgery
2. VMC Inc. *1 Seiji Yagi,1 Gotaro Shiota2
Purpose:
One of the initial treatments for extensive severe burns and gas gangrene is the elimination of sources of infection by disinfection. However, despite frequent disinfection, deterioration occurs in overall condition due to wound infection. Recently, reports have shown that ozone water is extremely effective in preventing hospital-acquired infections and disinfecting food. In the medical field, it has also been reported to be effective in preventing MRSA infection in the umbilical cords of newborns, and it is used in oral and ocular cleaning, suggesting safety in clinical use. We have already confirmed in clinical practice that ozone water causes a rapid decrease in redness, pain, and swelling in mild burns and pain in herpes zoster. From these experiences, ozone water is considered to be disinfectant water that not only has a bactericidal effect but also an anti-inflammatory and pain-relieving effect. Therefore, in this study, we used ozone water in three cases of severe burns, which are highly susceptible to severe infections, and compared it extensively with conventional treatment (povidone-iodine).
Method:
We used 4 ppm ozone water generated by a direct electrolytic ozone water generation device (manufactured by VMC Inc.*1) for burns. The water temperature was kept at a lukewarm level, and the disinfected area was covered or washed for about five minutes, then left to dry naturally or wiped with gauze. The effectiveness was evaluated over time by monitoring the healing condition of the wound, the infection of the wound, and an improvement in inflammation.
Result:
The disinfection effect that we aimed for was recognized as being equivalent or superior to the conventional method. Furthermore, to our surprise, a pain-relieving effect on the disinfected area was recognized compared to the conventional method, and a wound healing effect and improvement of autoimmune function were also confirmed in some cases. In addition, cases that previously had to be treated surgically with the conventional method were able to heal conservatively.
Discussion:
Ozone decomposes into oxygen, so it has low toxicity to cells. In addition, the sterilizing effect of ozone water is also useful in surgical diseases as well as ophthalmology and dentistry. However, its effect is limited to disinfection of the wound surface, and it is suggested that in cases where the source of infection is in the subcutaneous tissue, sufficient disinfection may not be obtained. In addition, the fact that the patients felt less pain means that the ozone water causes little tissue destruction. Therefore, it is considered to be useful for elderly people and patients with low immunity. And this could lead to more widespread use for cleaning bedsores and for other applications.
*1_Presently Mediplus Pharma, Inc.
Investigation of Bactericidal and Fungicidal Effects of Stabilized Ozone Gel
Mediplus Pharma, Inc. (Formerly VMC Corporation) Gotaro Shiota
Yoshiaki Maruyama, Kikumi Suzuki
Showa University School of Dentistry, Department of Prosthodontics, Akihiko Shiba
Tokyo Medical and Dental University Graduate School of Medical and Dental Sciences, Shiba Kiyoko, Okamura Noboru,
Chida Toshio
Division of Laboratory Medicine, Chiba University Hospital, Asako Kimura Read more
